ADAPTER AUTOMATE 25X13 SET 2

3'RACE PCR

: 2ul of undiluted cDNA, 2ul of 10 x diluted cDNA and 2ul of 100 x diluted cDNA in the PCR reaction. Set up the PCR as follows: 35.5ul pure water 5ul 10 x PCR buffer (Boehringer Mannheim) 5ul 500uM dNTPs (some enzymes require a higher

3'RACE PCR

.5. Use a serial dilution of the reverse transcription reaction: 2ul of undiluted cDNA, 2μl of 10 x diluted cDNA and 2ul of 100 x diluted cDNA in the PCR reaction. Set up the PCR as follows: 35.5μl pure water 5μl 10 x PCR buffer (Boehringer Mannheim

3'-RACE PCR

4. Mix and incubate at 37°C for 2 hours.   5. Use a serial dilution of the reverse transcription reaction: 2ul of undiluted cDNA, 2ul of 10 x diluted cDNA and 2ul of 100 x diluted cDNA in the PCR reaction. Set up the PCR as follows