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- Beckman Coulter贝克曼库尔特
- 美国
- B32766
- 2025年07月08日
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文献和实验: 2ul of undiluted cDNA, 2ul of 10 x diluted cDNA and 2ul of 100 x diluted cDNA in the PCR reaction. Set up the PCR as follows: 35.5ul pure water 5ul 10 x PCR buffer (Boehringer Mannheim) 5ul 500uM dNTPs (some enzymes require a higher
.5. Use a serial dilution of the reverse transcription reaction: 2ul of undiluted cDNA, 2μl of 10 x diluted cDNA and 2ul of 100 x diluted cDNA in the PCR reaction. Set up the PCR as follows: 35.5μl pure water 5μl 10 x PCR buffer (Boehringer Mannheim
4. Mix and incubate at 37°C for 2 hours. 5. Use a serial dilution of the reverse transcription reaction: 2ul of undiluted cDNA, 2ul of 10 x diluted cDNA and 2ul of 100 x diluted cDNA in the PCR reaction. Set up the PCR as follows
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