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文献和实验: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
Inhibition of Glycogen Synthase Kinase-3 Methods in Molecular Biology v468. chapter 5 Abstract There are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
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