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- Beckman Coulter贝克曼库尔特
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- 2025年07月15日
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文献和实验product is detected in the PCR process as a significant increase in fluorescence, and the lower the Ct value. Ct values are very reproducible in replicates because the threshold is picked to be in the exponential phase of the PCR, where there is a linear
In Vivo Luciferin Imaging Procedure
is plumbed to the anesthesia manifold located inside the imaging chamber. After the mice are fully anesthetized, they are transferred from the box to the nose cones attached to the manifold in the imaging chamber, the door is closed, and the “Acquire” button
denaturation of DNA will result in the inefficient use of the template in the first amplification cycle and, consequently, poor yield of PCR product. The annealing temperature may be estimated as 5°C lower than the melting temperature of the primer-template
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