BOTTLE GUIDE, LARGE 100mL

Hemacytometer Reference Guide

per square by 104 to get the number of cells per ml of the original cell suspension. Determine the grand total number of cells available for subculturing , multiply the number of cells per ml by the total number of mls

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using

Purification of Plasmid from 50 ml-culture

1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)