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文献和实验Spotted Microarray Hybridization and Scan
to a small volume, normally less than 5ml, do NOT overdry. Add 50ml Sigma ArrayHybTM Hybridization Buffer Heat mixture to 100°C 5 minutes, mix by pipetting up and down if necessary Spin for 5 minutes at 14,000 RPM
Water 700ml Store at room temperature. Wash 2 (1 litre) 20x SSPE 3ml PEG200 1.8ml Water 995ml Store at room temperature. Place 50ml of Wash 1 in a 50ml sterile tube
In Situ HYBRIDIZATION TO TISSUE SECTIONS
glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108
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