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文献和实验The Polymerase Chain Reaction (PCR)
purification issues. • Mullis properly exploited amplification.The Basics of PCR Cycling • 30–35 cycles each comprising: – denaturation (95°C),30 sec. – annealing (55–60°C),30 sec. – extension (72°C),time depends on product size. What’s in the Reaction?
vol 687 Chapter 2 采用DNA聚合酶融合技术进行长片段PCR扩增 (精华)
several disadvantages,including: (1) low fidelity which leads to a high percentageof error-containing clones (e.g., 100% of 10 kb fragmentsare expected to contain at least one error; Table 1); and (2) longPCR extension times that can damage DNA and lead to run
的BLOCK-iT™产品产品应用优势 产 品 应 用 优 势 BLOCK-iT™ Dicer RNAi Transfection Kit
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