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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
. 4:21-25). The formula in Davis and de Serres, p. 86, incorporates this change. The trace elements used for Vogel''s medium N are suitable for SC at 0.1 ml per liter final volume; there is no need for a separate formula. Russo, et al. (1985 N.N. 32
, stained with propidium iodide (P1304, P3566, P21493) and visualized by confocal laser-scanning microscopy. This photomicrograph clearly shows that the green-fluorescent lectin staining outlines the apoptotic surface blebs, whereas the red-fluorescent
of S. mansoni (Spotila et al. 1989; Hirai et al. 1989). This protocol uses the PRINS reaction kit from Boehringer Mannheim, together with Taq DNA polymerase from GibcoBRL. Reagents from other manufacturers may work equally
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