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文献和实验in a loss of viability. If a CO2 incubator is not available gas the flasks for 1-2 minutes with 5% CO2 in 95% air filtered through a 0.25m filter. For some cultures it is necessary to subculture before confluence is reached in order to maintain
Subculture of Suspension Cell Lines
is not available gas the flasks for 1-2 minutes with 5% CO2 in 95% air filtered through a 0.25m filter. For some cultures it is necessary to subculture before confluence is reached in order to maintain their characteristics e.g. the contact inhibition of NIH
Subculture of Suspension Cell Lines
. Do not use an incubator to thaw cell cultures since the rate of thawing achieved is too slow resulting in a loss of viability. If a CO2 incubator is not available gas the flasks for 1-2 minutes with 5% CO2 in 95% air filtered through a 0.25m filter
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