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文献和实验The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Reverse transcription (RT) Materials required RT-PCR kit (Gibco) Primers (300 nM each) PCR thermocycler Microfuge Microfuge 0.5 ml thin walled PCR tubes Thermocycler Ð Caution
up termination mixes for each template!1.Label four 1.5 ml microfuge tubes “ddA,ddC,ddG,ddT” for the respective termination mixes. 2.To each tube,add the components indicated below to prepare the dNTP/Cy5 ddNTP termination mixes,beginning with the water
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