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文献和实验Construction and Characterization of Adenovirus Vectors
) Tubes (polystyrene, conical, 15-mL) Tubes (polystyrene, round-bottom, 5-mL) Tubes (ultracentrifuge, Quick-Seal, 16- x 76-mm) (Beckman 344322) Tubes (ultracentrifuge, 14- x 89-mm) Ultracentrifuge Vortexer Water baths
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Access RT-PCR System (Promega)
: _____________________; OD 280: _____________________________260/280: _______________________Calculate mg/mL of RNA in your samples:(OD 260 X dilution factor X 40)/1000 OR OD 260 X 2 = ___________________ ug/uLmL to deliver 0.1 mg RNA __________________________2. Determine
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