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文献和实验Sample preparation (preparative gels)
Human samples CEC (Colon epithelial cell): Within a maximum of 30 min, a large right or left colon tissue sample (5 x 7 cm) was prepared on ice in the operating room, rinsed with cooled phosphate buffer saline, pH 7.2 to further remove
Sample preparation (analytical gels)
protein solubilization. The SWISS-2DPAGE samples were prepared as followed: Human samples CEC (Colon epithelial cell): Within a maximum of 30 min, a large right or left colon tissue sample (5 x 7 cm) was prepared on ice in the operating room
Sample preparation (analytical gels)
solubilization.The SWISS-2DPAGE samples were prepared as followed: Human samples CEC (Colon epithelial cell): Within a maximum of 30 min,a large right or left colon tissue sample (5 x 7 cm)was prepared on ice in the operating room,rinsed with cooled phosphate
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