- ￥12133
- Beckman Coulter贝克曼库尔特
- 美国
- C35992
- 2025年07月15日
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文献和实验it is a good idea. 17. Yield: 0.8 to 1.1 mg of pure total RNA per ml of packed worms. These preps will be contaminated to some extent with RNA from E. coli that were in the starting material. The proportion of E. coli RNA in the prep can be assessed
秀丽线虫RNA提取--C. elegans RNA prep
秀丽线虫RNA提取 C. elegans RNA prep by Michael Koelle and Tory Herman, adapted from Sambrook et al., "Molecular Cloning" C. elegans RNA preps that have been widely used previously involved fairly slow lysis
the cell pellets in a total of 70 ml of GET/Lysozyme solution (35 ml for each bottle) by gently teasing the pellet with a spatula and incubate for 10 minutes at room temperature. (Note: Do not vortex the lysate at any time because this may shear
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