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文献和实验实验试剂 Sterile water (high purity, RNase-free) 5 x SuperScript RTase buffer (Life Technologies) 100mM DTT 2.5mM dNTPs RNase inhibitor (Pharmacia) SuperScript RTase
The protocol given uses SuperScript II reverse transcriptase (Life Technologies) and Taq polymerase from Boehringer Mannheim. You will need to adjust the reagents according to the enzymes you use. Reagents: Sterile water (high purity, RNase-free)
E-Z 96 X-press Plasmid Vacuum Protocol
collection plate as a support to give the collection plate a proper position. 15. Add 100-150 μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. Apply maximum vacuum for 5-10
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