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文献和实验free shotgun sequencing library from miniprep BAC DNA
with gelase ( Gelase TM Agarose Gel-Digesting Properation, Epicentre Technologies, #09200) or QIAquick Gel Extraction Kit ( Qiagen, # 28704) , or GeneClean Kit for Purification of DNA from agarose gel ( Bio 101, #3105). 9.Sonication and end-repair DNA
freeshotgun sequencing library from miniprep BAC DNA
size and with/without Not 1 cut site in the insert of individual BAC clone with gelase ( Gelase TM Agarose Gel-Digesting Properation, Epicentre Technologies, #09200) or QIAquick Gel Extraction Kit ( Qiagen, # 28704) , or GeneClean Kit for Purification
Marcantonio Lab Protocol Manual
containing 498 ul of DD-H2O. Mix this and transfer it to a clean cuvette. Pipet 500 ul of DD-H2O into another cuvette that will be used as a reference sample. 3) Place the reference cuvette into the instrument, close the lid, and set the wavelength to 260
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