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of template DNA. The 25 l volume " reactions were overlaid with 50 l light mineral oil and denatured at 95 C for 5 min. The reactions were processed through 75 cycles of 94 "for 10 s, 36 C for 10 s, and 72 C for 2 min. Either a PTC 100 " thermocycler (MJ
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ACCUMULATOR, OIL(Service)
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