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文献和实验Myosin Light Chain Preparation
the 8 liter and the 28 liter beakers. 5.Put ETOH in the cold room. 6.Refrigerate or equilibrate the DE52 to be used. 7.Find parts to and assemble the 5½ cm ID column. 8.Make sure there is plenty of cold water in the cold room. 9.Thaw 8 M urea. Solutions
Use of Channelrhodopsin for Activation of CNS Neurons
) Heat shrink tubing (both 3/32‐in. and 1/8‐in. diameter) Blue light laser with fiber coupler (e.g., 150 mW, 473‐nm diode‐pumped solid‐state continuous wave laser
7, or M13 primers) For lab stocks record on each tube: Plasmid name, plasmid ID number (red ink), your initials, date of plasmid prep or lab book page number, DNA concentration. Consider reprepping the DNA if the A260 /A280 is less than 1.88
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