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文献和实验2-D Polyacrylamide Gel Electrophoresis
. 3.Vortex vigorously for ~15 sec. 4.Incubate at RT for 5 min. 5.Vortex again. 6.Spin down for 3 min to pellet the tissue. 7.Remove xylenes. 8.Add 1 ml xylenes. 9.Vortex 5-10 sec. 10.Spin down. 11.Remove xylenes. 12.Speed vacuum the sample
Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
. Support protocols describe porphyrin synthesis, sensor preparation, and sensor calibration. GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
Measurement of Chloride Movement in Neuronal Preparations
(Whatman GF/C or Schleicher & Schuell no. 30) 1‐liter vacuum flask with Tygon tubing and vacuum pump (do not use a vacuum line)
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