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文献和实验秀丽线虫RNA提取--C. elegans RNA prep
. 5. Pour the mixture into a 28 ml polycarbonate Oak Ridge tube. Spin in the 70Ti ultracentrifuge rotor for 20 minutes at 30K RPM at 20° to pellet debris (and any metallic stuff from the polytron). Pour off the supernatant into a clean 50 ml
100 ml, sterile filter to remove particulate matter pour into a bottle, and carefully mark the level of the meniscus Add DEPC to 0.1%, shake to dissolve DEPC Let stand overnight at 37o Autoclave 20 minutes After autoclaving
Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
) Three‐necked flasks with condensers Rubber septa (+15.9 mm) 500‐mL tight, high‐pressure tubes (schott
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