- ￥5335
- Beckman Coulter贝克曼库尔特
- 美国
- C31600
- 2025年07月11日
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文献和实验µl of 10% ammonium persulfate to 75 ml of Gel-mix-6. Mix by inversion. Hold the nozzle of the bottle at the corner of the gel cast. Hold the gel cast at a 45o angle to the bench and pour the gel between the plates. If bubbles get trapped
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Endothelial Cell Systems - Instructions For Use
-5022)1 x 100 ml bottle Trypsin/EDTA Solution (CC-5012) 1 x 100 ml bottle Trypsin Neutralizing Solution (CC-5002) 1 x 100 ml bottle NOTE: If you use a different Clonetics® medium, see Appendix A, Endothelial Cell Media Endothelial Cell Systems Media
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