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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
in the fermentor vessel and check that it is pressurized to 2 atm. using the bicycle pump. After sterilization, check the pH of the media with a pH meter and adjust the pH zero point on fermentor if necessary. If you use the pH probe, remove it from the fermentor
Aggregation of ES Cells and Eight Cell Stage Embryos
and 3 cc syringe (filled) to run a small amount of silicone (Sigmacote) through the inside of a piece of capillary glass, allowing excess Sigmacote to drip into a 10ml tube. This is repeated with each piece of glass and the glass placed in a measuring
Plant DNA methylation analysis by bisulfite genomic sequencing
above; Overlay 3 drops (~100µl) mineral oil; Incubate in a PCR cycler over night: 2 minutes at 95°C 1 time, 2 hours at 75°C followed by 1 minute at 95°C 9 times, hold at 75°C. Some precipitate will form, but this will not interfere with later
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