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文献和实验DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
DNeasy® 96 Plant Protocol for Isolation of DNA from Fresh Plant Leaves Using the Mixer Mill MM 300 一、Important notes before starting (使用前的重要注释) 1、Take time to familiarize yourself with the Mixer Mill MM 300 and the Centrifuge 4-15C/ Centrifuge 4K15C
Preparation of Embryonic Extract
. Keep the beaker on ice. Alternatively, a Dounce homogenizer with coarse fitting can be used. Homogenize for 10-15 strokes. 13. Transfer no more than 25 ml of the homogenate to a sterile 50 ml conical centrifuge tube. Read volume. 14. Dilute 1:1 with Hank's
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
into each microcentrifuge tube (~500 islets/tube/mouse). Allow islets to settle to the bottom of the microcentrifuge tubes. Draw one islet pellet into a P200 pipetman (set at 130ul) using a straight thin-wall pipet tip. Place a silicone tube adapter
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