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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验步骤 1. Place a empty, uncapped 50 ml conical tube into a 50ml tube holder. 2. Add 1-6 ml blood sample to 50ml tube. 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix
Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
实验步骤 1. Place two empty, uncapped 50 ml conical tubes into a 50ml tube holder. 2. Equally divide the blood sample and transfer the sample into twp 50 ml tubes. For example: For 7ml blood, transfer 3.5ml blood into each tube
Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
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