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HANDLE AY, ROTOR TY-80TI(Service)
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文献和实验相关实验
秀丽线虫RNA提取--C. elegans RNA prep
to use freshly prepared worms, since these can be lysed most quickly, but a flash frozen pellet that was stored at -80° is fine. Do not process more than one 5 ml aliquot of worm suspension at a time; this will entirely fill the SW28.1 rotor for the later
metal? chunks; don't worry, you can spin these out.) 4. Add sarcosyl to 0.5 % (using the RNase free 20% solution) and mix. 5. Pour the mixture into a 28 ml polycarbonate Oak Ridge tube. Spin in the 70Ti ultracentrifuge rotor for 20 minutes at 30
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HANDLE AY, ROTOR TY-80TI(Service)
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