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文献和实验-throughput purification of genomic DNA and total RNA from up to 96 samples. DNA and RNA are simultaneously purified from each cell or tissue sample. Since there is no need to divide each sample into two for separate purification procedures, maximum yields
96 Well Mating Protocol platewise
is between the H1H2 recombination regions which lie just inside two I-SceI cut sites (Sce1-H1-Cassette- 96 well Mating Protocol Preparation: 1. Streak fresh donor plasmid on LB proper antibiotic IPTG/XGAL plate (donor F’ strain contains lac , it will be blue
96 Well Mating Protocol platewise
just inside two I-SceI cut sites (Sce1-H1-Cassette- 96 well Mating Protocol Preparation:1. Streak fresh donor plasmid on LB + proper antibiotic +IPTG/XGAL plate (donor F’ strain contains lac +, it will be blue on the XGAL plate2. Streak recipient plasmid (pMSCV) on LB
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