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文献和实验. F. Biomek-automated modified-Eperon isolation procedure for single-stranded M13 DNA This semi-automated method is a modification of a previously reported procedure (24,25), and allowed the simultaneous isolation of 48 single-stranded
In Situ Hybridization Using Digoxigenin Labeled Probes
. The staining reaction can be observed by blotting off excess solution and examining the slide under the microscope using a low magnification phase contrast lens (e.g. 16x) 9) Stop the reaction by dipping the slides in distilled water three times
3 . Smaller volumes can be prepared as needed. 1. In a large vessel, add 9 l of H2 0. Dissolve enough powdered MEM to prepare 10 l. Add 30 g glucose, 15 mg methionine, 52 mg leucine and 725 mg lysine-HCl so that there will be 4, 0.1, 0.1 and 1.0 times
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