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- Beckman Coulter贝克曼库尔特
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- 2025年07月08日
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文献和实验. F. Biomek-automated modified-Eperon isolation procedure for single-stranded M13 DNA This semi-automated method is a modification of a previously reported procedure (24,25), and allowed the simultaneous isolation of 48 single-stranded
using the squirt bottle. Immediately place the potted plant into a Magenta box with lid. Return the plants to the culture room (23!C, 24 hr light). Within a day or two, the stems should straighten up and start to grow. As quickly as possible
Western blotting using ECL reagent
, incubate for 15 minutes, then repeat 2 x at 5 minutes each. Use a lot of buffer.3. Transfer the membrane to a lid of 96-well microtiter plate or similar low volume container. Incubate with first antibody using recommenced dilution in TBS + 1.5% gelatin
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