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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user 1. Centrifuge capable of at least 3,000-5,000 x g 2. Rotor adapter for 96
Transformation of E. coli by Electroporation
number of microcentrifuge tubes. Place the required number of Micro-electroporation Chambers on ice. Fill the temperature control compartment of the Chamber Safe with ~250 ml of ice-water slurry and place the Chamber Rack in the Chamber Safe. 2. Thaw
several times to ensure temperature consistency. Adjust the temperature setting to 80°C. for 5 minutes. Remove the container from the microwave and dump paraffin into the paraffin pot that is set at a temperature of 84°C. Remove the processing rack
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