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文献和实验Quick and Easy Isolation of Genomic DNA from Yeast
should be resuspended in a 10 µl volume, because the yield will be smaller. 0.25 µl RNase cocktail should be added to the samples used for Southern blot hybridization (final concentration 0.125 U RNAse A, 5 U RNase T1). Reagents Harju- Buffer � 2%
NuPAGE Gel Electrophoresis 蛋白电泳
BufferVoltage: 200V constantExpected Current: Start: 110-125 mA per gel; End: 70-80 mA per gelApprox.Run Time: 35 minutes10% Bis-Tris Gel with MOPS Running BufferVoltage: 200 V constantExpected Current: Start: 110-115 mA per gel; End: 60-70 mA per gelApprox.Run
: 110-125 mA per gel; End: 70-80 mA per gel Approx. Run Time : 35 minutes 10% Bis-Tris Gel with MOPS Running Buffer Voltage: 200 V constant Expected Current : Start: 110-115 mA per gel; End: 60-70 mA per gel
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