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- Beckman Coulter贝克曼库尔特
- 美国
- 364219
- 2025年07月13日
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文献和实验of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
pellet in 1 mL of nuclei lysis buffer for ChIP supplemented with protease inhibitors and incubate for 10 min on ice. 8. Proceed with sonication. Ensure that the samples do not foam and are kept as cold as possible. 在完成1-8步骤后用 1. mix 20 ul
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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