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- Beckman Coulter贝克曼库尔特
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- 2025年07月13日
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
in each well. Using a multichannel pipette colonies are dispersed by pipetting up and down 40x in a circular motion being careful not to make bubbles as these cause cell death, check that colonies are dispersed. Cells are then transferred to a 96 well tray
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
that colonies are dispersed. Cells are then transferred to a 96 well tray, containing Neo PMEF's. This process is repeated until all clonies are picked. After 3-4 days clones should be ready for splitting and freezing down. Splitting and Freezing
Viewing and Editing Assembled Sequences Using Consed
Necessary Resources Hardware Workstation with ∼256 MB RAMConsed/Autofinish requires ∼256 MB of memory for most BACs. Larger projects (or projects
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