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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验步骤 1. Place a empty, uncapped 50 ml conical tube into a 50ml tube holder. 2. Add 1-6 ml blood sample to 50ml tube. 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix
Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
实验步骤 1. Place two empty, uncapped 50 ml conical tubes into a 50ml tube holder. 2. Equally divide the blood sample and transfer the sample into twp 50 ml tubes. For example: For 7ml blood, transfer 3.5ml blood into each tube
Sephadex G-50 spun column purification
. 1) Plug a 1ml syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place the syringe barrel into a 10ml centrifuge tube and spin at 3,000g
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