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文献和实验Separation of sea urchin blastomeres
Enough eggs to cover the bottom of a 50 ml beaker were transfered along with 10 ml of 10mM p-aminobenzoic acid in ASW. 50 ml of sperm were diluted in 5 ml of ASW. 2 drops were used to fertilize eggs. The presence of the fertilization envelope
Lipoprotein Isolation - First week
100 ml beaker and add 25 µl of 200 mM PMSF Caution! Highly poisonous! picture ). Make sure no insect saliva drips into the beaker (have Kleenex ready to wipe off saliva). 3. Gently inject a few hundred microlitres of PBS
Lipoprotein Isolation - First week
3 Lyophilization. Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean 100 ml beaker and add 25 µl of 200 mM PMSF
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