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文献和实验Lipoprotein Isolation - First week
100 ml beaker and add 25 µl of 200 mM PMSF Caution! Highly poisonous! picture ). Make sure no insect saliva drips into the beaker (have Kleenex ready to wipe off saliva). 3. Gently inject a few hundred microlitres of PBS
Lipoprotein Isolation - First week
3 Lyophilization. Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean 100 ml beaker and add 25 µl of 200 mM PMSF
was performed in a glass 100-ml beaker as follows. At intensive stirring, 20 drops of NaOH solution (5 M) were added to AgNO3 solution (500 mg) in Milli-Q water (60 ml), and dark brown precipitate formed immediately. Approximately 2 ml of 30% NH4 OH solution
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