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- Beckman Coulter贝克曼库尔特
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- 2025年07月14日
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文献和实验Appendix H: Radioactive tracers
a flash of light for each radiation particle it encounters. The counter than converts light energy (either as counts of flashes, or as an integrated light intensity) to an electrical measure calibrated as either direct counts or counts per minute (CPM
Analys of Genomic DNA by Southe
the cut DNA in the desired volume. You can try using 10 units of restriction enzyme per µg of DNA and digesting overnight (note: this is only useful for stable restriction enzymes- the Biolabs catalog has a table showing the stability of various enzymes
Analys of Genomic DNA by Southe
(these are repetitive sequences). To ensure complete digests, dilute the digest into a larger volume (thus diluting the enzyme inhibitors) and then precipitate and resuspend the cut DNA in the desired volume. You can try using 10 units of restriction enzyme per µg
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