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文献和实验v468 Chapter 9 自身启动子报告基因验证转录靶点
of the reaction mix to electrophoresis on a 1%(w/v) agarose gel to check for correct size of the amplicon. 4. For subsequent purification of the amplicon from the reactionmix, use the Qiagen PCR Purification Kit according tothe user manual, and elute in 30 μL
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
., Wagenaar, E.,Parren, P., Weigel, D., and Nusse R. (1987) The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell 50 , 649–657. 3. McMahon, A. P. and Moon, R. T. (1989) Ectopic expression
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