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文献和实验) for 1 minute to dry the column. 8) Place the column in a clean 1.5 mL microcentrifuge tube and add 10-20ul Elution Buffer (10mM Tris, pH8.5 ). Stand for 1-2 minute and centrifuge at maximal speed ($13,000 x g) for 1 minute to elute DNA.
reactions. They are right about this. Place the QIAprep column in a clean 1.5 ml microcentrifuge tube. To elute DNA, add 50 μl Buffer EB (10 mM Tris・Cl, pH 8.5) or water to the center of each QIAprep spin column, let stand for 1 min, and centrifuge
E.Z.N.A. Cycle-Pure Kit Spin Protocol
. 9. Place HiBind® DNA column into a clean 1.5ml microcentrifuge tube. Add 30-50ul (depending on desired concentration of final product) of Elution Buffer (10mM Tris, pH8.5) or water directly onto the column matrix and centrifuge for 1 min at 13,000
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