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文献和实验Phosphoamino acid analysis:Mark Kamps's method
virus phosphorylates tyrosine.Proc Natl Acad Sci U S A 1980 77:1311-1315. Kamps,M.P.,and Sefton,B.M.(1989)Acid and base hydrolysis of phosphoproteins bound to immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins.Anal Biochem
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008)Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
CATHY'S NO BOIL YEAST EXTRACTS
CATHY'S NO BOIL YEAST EXTRACTS 1.Cells to mid log/ need ~ 5OD.Spin down and wash w/ 1ml H2O 2.Transfer to 1.5ml round bottom screw cap tube 3.add 200μl SDS sample buffer+ (5% BME,50mM NaF,1mM PMSF)-add to stock just before use; + 200μl glass
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