- ￥125 - 1245
- Watson
- 1332-015S
- 日本
- 2026年01月14日
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- 库存：
现货
- 保修期：
见包装
- 现货状态：
现货
- 供应商：
上海然其生物科技有限公司
- 规格：
48支/包/10包/箱
| 规格： | 48支/包 | 产品价格： | ￥125.0 |
|---|---|---|---|
| 规格： | 10包/箱 | 产品价格： | ￥1245.0 |
Watson 1332-015S 15mL Centrifuge Tube (Non-Racked) Product Introduction
Core Specifications
| Item | Details |
|---|---|
| Model | 1332-015S |
| Capacity | 15mL |
| Bottom Type | Conical bottom |
| Cap Type | White triple-thread half-plug seal cap (with sealing point prompt) |
| Material | Medical-grade polypropylene (PP), high transparency |
| Sterility | Gamma-irradiated sterilization, SAL 10⁻⁶ |
| Contamination Control | RNase/DNase/Human-DNA free, endotoxin ≤0.05EU/mL |
| Max Centrifugal Force | 18,000×g (14,100 RPM, 20°C, 12mL loading volume) |
| Graduation | Precision molded scale, up to 0.5mL at the cone bottom, not easy to fade |
| Packaging | 48 tubes/bag, 10 bags/case (480 tubes total), no rack |
| Origin | Made in Kobe, Japan |
Key Features & Advantages
- Superior Sealing Performance: The triple-thread and half-plug seal structure, combined with the sealing point prompt (audible click when the cap is in place), effectively prevents liquid leakage and sample contamination during centrifugation and transportationWATSON.
- Low Contamination Risk: Produced in a clean room, it has passed strict detection to ensure no RNase, DNase and human DNA residues, and low endotoxin content, which is suitable for nucleic acid extraction, cell separation and other experiments.
- High Centrifugal Resistance: It can withstand a maximum centrifugal force of 18,000×g, which is suitable for most high-speed centrifugation applications in laboratoriesWATSON.
- User-Friendly Design: The tube body has a frosted area and the cap has a wide writing space for easy marking; the precision scale facilitates accurate liquid volume controlWATSON.
- Stable Quality: Each batch is accompanied by a COA quality inspection report to ensure consistent performance indicators.
Typical Applications
- Molecular biology experiments: Nucleic acid purification, PCR sample preparation, plasmid extraction.
- Cell culture: Cell separation, supernatant collection, medium change.
- Protein research: Protein purification, enzyme reaction system preparation.
- Clinical and pharmaceutical research: Sample pretreatment, reagent distribution, quality control experiments.
Usage Notes
- When screwing the cap, stop when you hear a click to ensure the best sealing effect and avoid excessive screwing damage to the tube mouthWATSON.
- It is recommended that the liquid loading volume does not exceed 12mL to prevent liquid overflow during centrifugationWATSON.
- Avoid contact with strong corrosive chemicals to prevent damage to the tube body.
- Store in a dry and cool environment, away from direct sunlight.
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文献和实验argeted Mutagenesis Using RNA-guided Endonucleases in MossesBio-protocol, 2017, 7(12): e2359. DOI: 10.21769/BioProtoc.2359Toshihisa, K., & Hitoshi, S. (2017). Targeted Mutagenesis Using RNA-guided Endonucleases in Mosses. Bio-protocol, 7(12), e2359. 苔藓 RNA 引导核酸酶靶向诱变，用于细胞收集与核酸纯化
的DNA分布在不同的区带。 实验结果表明：在全部由15N标记的培养基中得到的15N�NA显示为一条重密度带位于 离心管 的管底。当转入14N标记的培养基中繁殖后第一代，得到了一条中密度带，这是15N�NA和14N-DNA的杂交分子。第二代有中密度带及低密度带两个区带，这表明它们分别为15N14N－DNA和14N14N-DNA。随着以后在14N培养基中培养代数的增加，低密度带增强，而中密度带逐渐减弱，离心结束后，从管底到管口，CsCl溶液密度分布从高到低形成密度
)密度梯度离心法观察DNA所处的位置。由于15N�NA的密度比普通DNA(14N－DNA)的密度大，在氯化铯密度梯度离心(density gradient centrifugation)时，两种密度不同的DNA分布在不同的区带。 实验结果表明：在全部由15N标记的培养基中得到的15N�NA显示为一条重密度带位于 离心管 的管底。当转入14N标记的培养基中繁殖后第一代，得到了一条中密度带，这是15N�NA和14N-DNA的杂交分子。第二代有中密度带
Hoechest 33258荧光染料37℃孵育15~30分钟，于荧光显微镜下观察。凋亡细胞由于染色质固缩，细胞核呈致密浓染，或呈碎块状致密浓染。操作比较容易的。altbenair: 想做肝癌细胞 hepG2。看过很多帖子 还有文献上的方法，但是都不太具体大都是这样：固定（福尔马林4%），pbs洗三遍，加hoechst孵育1H，将细胞悬液滴于载玻片上荧光显微镜检测。细胞悬液怎么做？我要做贴壁细胞 用胰酶消化？drake015: .1．贴壁细胞 ，让细胞自己在盖玻片上爬片。操作如下：A. 取普通洁净盖玻片于70
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