CATHY'S NO BOIL YEAST EXTRACTS

CATHY'S NO BOIL YEAST EXTRACTS

1.Cells to mid log/ need ~ 5OD.Spin down and wash w/ 1ml H₂O

2.Transfer to 1.5ml round bottom screw cap tube

3.add 200μl SDS sample buffer⁺ (5% BME,50mM NaF,1mM PMSF)-add to stock just before use; + 200μl glass beads

4.Beat 90sec (setting 9)in cold room and spin down 2',4℃.

Sample buffer: 4% SDS 5ml+

80mM Tris Cl6.8

Final 10% glycerol

10mM EDTA

0.2% BFB