货号：VWRVM152 AMRESCO GLYCEROL

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

of interestSodium citrate (50 mM, pH 6.0)TPCK-modified trypsin (sequence-grade)EquipmentCompact reaction columns (CRC) and filters (35-µm pore size)Incubator (slow rotation, e.g., rotating wheel)MALDI mass spectrometer (e.g., the Voyager DE-STR) or equivalentThe

顽拗性植物组织的蛋白质组学研究（苯酚法提取蛋白质）

多糖蛋白。 苯酚提取法有高度的清洁能力。它能够作为一种分离剂，降低蛋白质和其他分子间的相互影响。苯酚提取法最主要的不足在于它时间消耗过长（至少 6 h) ，另外苯酚和甲醇都是有毒的。2. 材料 2.1 苯酚 饱和 Tris-HCl 缓冲液，pH 6. 6/7.9  ( Amresco- Interchim， biotechnology  grade) 。 2.2 提取缓冲液 准备一份含有 500 mmol/L Tris-HCl 缓冲液、500 mmol/L EDTA、700 mmol

Cell‐Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening

maintenance medium (see recipe ) Huh7 cell maintenance medium with 1% (v/v) dimethyl sulfoxide (DMSO; tissue culture grade)