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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见瓶身
- 保质期:
/
- 英文名:
/
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
7365-45-9
- 规格:
100 ml
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文献和实验. The supernatant was aspirated, and the pellet resuspended in 5ml of warm sterile growth medium. This growth medium consisted of Medium 199(with 5% HEPES, Earle’s Salts, L-glutamine, L-glutamine tryptophan and phenol red) containing added Foetal Calf serum (20
sucrose 20mM Tris pH 7.2 10mM b-glycerophosphate 1mM EDTA 1mM EGTA 0.1% b-mercaptoethanol For most applications, use the following protease and phosphatase inhibitors ([final;stock]): NaF (1mM; 1M), Na3VO4 (1mM; 0.1M
Procedures for In Vitro Production of Bovine Embryos
for one week only.Stock 20: PO 4 . Dissolve 0.235 g NaH2 PO4 +H2 O in 50 mL water. Sterile filter and store at 4°C.Stock 21: 1 M HEPES. Add 119 g of HEPES to 400 mL water. Adjust pH to 7.0 and bring volume up to 500 mL. Sterile
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