货号：VWRVM153 AMRESCO GLYCEROL

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

of interestSodium citrate (50 mM, pH 6.0)TPCK-modified trypsin (sequence-grade)EquipmentCompact reaction columns (CRC) and filters (35-µm pore size)Incubator (slow rotation, e.g., rotating wheel)MALDI mass spectrometer (e.g., the Voyager DE-STR) or equivalentThe

免疫沉淀-蛋白印迹法

(HRP)-conjugated IgG; Rabbit Anti-Mouse horseradish peroxidase were from Perbio Science UK Ltd (Cheshire, UK).Heparin (sodium salt Grade A), glycerol, methanol, Bromophenol Blue (sultone form), 2-mercaptoethanol, glycine, leupeptin, aprotenin, NaCl

ChIP Assay Protocol

7.5,83 ml nuclease-free water •Protease inhibitors (200mM PMSF in isopropanol,2 mg/ml Aprotinin,2 mg/ml Leupeptin)•37% reagent grade Formaldehyde•1.25 M glycine•1% Agarose gel/EtBr1.Transfect,induce,or grow 1 to 5x106 cells in 10 cm dish.2.Rinse cells