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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD9
- 级别:
CD9 Antibody
- 目录编号:
CD9 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD9 Antibody
- 抗体英文名:
anti-rat CD9 Antibody-APC
- 抗体名:
CD9 Antibody
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
大鼠
- 免疫原:
CD9 Antibody
- 亚型:
CD9 Antibody
- 形态:
CD9 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/mL
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2700.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells,including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found toparticipate in forming a large molecular cell complex with other membrane proteins, such asMHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker ofmarginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largelydepend upon its associated molecules on different cells.
Verified Reactivity :Rat
Antibody Type :Monoclonal
Host Species :Mouse
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation :The antibody was purified by affinity chromatography and conjugated with APC under optimalconditions.
Concentration :0.2 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cellsin 100 µL volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser :Red Laser (633 nm)
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文献和实验CD9 分子 CD9 常用单克隆抗体或代号:PHN200,FMC56 主要表达细胞:Pt, Pre―B, M, Eo, Ba,Meg[Pt] 分子质量(kDa)和结构:Zp24(TM4―SF) 功 能:血小板凝集和活化,可能参与前B细胞黏附和信号转导;肥大细胞上CD9是lLl6受体 CD9 May mediate platelet activation
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗,这种抗体有单抗的吧? 用于FCM: 1:20-100,这是说用于流式的稀释倍数,是不是稀释倍数越小越好呢? 同型对照抗体是不是必须要做的?如何选择阴型同型对照抗体呢! 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助! freecell 抗体的稀释倍数是需要优化










