Description :Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved intranscriptional activation, chromatin decondensation, and chromosome compaction during celldivision, by the action of Aurora kinase and NIMA kinases.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Modified synthetic peptide conjugated to KLH
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Application Notes :The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residueand has been shown to be useful for Western blotting.