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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
of labelings: At the recommended reagent concentrations and volumes, this kit contains sufficient material to perform approximately 200 assays using flow cytometry. Approximate fluorescence excitation/emission maxima: calcein violet 400/452 nm; aqua
Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
were labeled with YO-PRO-1 dye (green) (Y3603). Necrotic cells were detected with propidium iodide (red) 10 µM喜树碱诱导的Jurkat cells(慢淋。G1检测点缺陷细胞) 凋亡。细胞然后经 Vybrant Apoptosis Assay Kit #4 (V13243).处理。凋亡细胞核被YO-PRO-1绿染,坏死细胞被pi红染。 Jurkat human T-cell leukemia
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