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- 2026年05月29日
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文献和实验CellTrace CFSE Cell Proliferation Kit
in phosphate-buffered saline (PBS) or other suitable buffer to the desired working concentration (0.5–25 μM). Resuspend the cells gently in prewarmed (37°C) PBS containing the probe (prepared in previous step). Incubate the cells for 15 min
Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate
of new lead structures. In this chapter, we describe two dengue virus protease assays based on an internally quenched, high-affinity F�rster resonance energy transfer (FRET) substrate (K m = 105 μM). A fluorimetric assay using a microtiter fluorescence
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
the instructions provided with the kit. Note: Because the CytoTune™ Sendai reprogramming vectors are based on SeV, which is an RNA virus, reverse transcription is required for detecting the presence of the SeV genome in your reprogrammed cells. c
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