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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
TCR vβ8.3
- 级别:
TCR vβ8.3 Antibody
- 目录编号:
TCR vβ8.3 Antibody
- 克隆性:
单克隆
- 抗原来源:
TCR vβ8.3 Antibody
- 保质期:
TCR vβ8.3 Antibody
- 抗体英文名:
Purified anti-mouse TCR vβ8.3 Antibody
- 抗体名:
TCR vβ8.3 Antibody
- 标记物:
仓鼠
- 宿主:
仓鼠
- 适应物种:
小鼠
- 免疫原:
TCR vβ8.3 Antibody
- 亚型:
TCR vβ8.3 Antibody
- 形态:
TCR vβ8.3 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
500 µg
Description :The TCR Vβ8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. 1B3.3 reacts withVβ8.3 only, but not Vβ8.1 or Vβ8.2. Most mouse strains have about 20% Vβ8+ T cells in theperiphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrchaplotype) do not express Vβ8 due to a gene deletion at the Vβ8 loci. Exogenoussuperantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ8 andselectively eliminate those T cells in vivo.
Verified Reactivity :Mouse
Antibody Type :Monoclona
Host Species :Armenian Hamster
Immunogen :3.L2 T Cell Clone Expressing the vβ8.3 TCR
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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文献和实验1. Kersh G, et al. 1998. J. Immunol. 161:585.
,T 和 B 细胞在免疫系统中展现两种不同功能的识别机制。T 细胞识别细胞膜表面 MHC I 或 II 类分子呈递的抗原肽来识别细胞。而 B 细胞没有选择识别机制。近几年,TCR 样抗体研发工作取得较大进展,许多研究团队都具备制作该抗体能力,研发出了许多针对肿瘤和病毒 T 细胞表位的 TCR 样抗体。能够获得被 T 细胞识别的,纯化后自然折叠 MHC-短肽复合物,是 TCR 样抗体制备关键。MHC-短肽复合物可以通过大肠杆菌表达获得。大肠杆菌分别表达 MHC 重链和β-球蛋白,在体外折叠后,与短肽
分抗体的运输条件:一般的运输过程需要1~2周的时间,所以是在低温4℃的条件下来完成的。 4℃运输最主要的目的是为了避免反复冻融对抗体活性的损害(如果使用干冰运输,那么收到时抗体是冻起来的,为了分装就需要解冻,这就多了一次冻融的过程),所以运输过程中绝对避免干冰运输。 (5)关于叠氮化钠(sodium azide)的使用: 为了防止微生物污染,叠氮化钠经常被加入到抗体中(终浓度0.02% (w/v))。ProSci的绝大部分抗体已经含有了0.02%
the sample briefly and load onto your SDS-PAGE gel.Add Dithiothreitol (DTT) or β-mercaptoethanol (2-ME) to the Trident Sample Buffer before use to reduce proteins, if necessary.电泳和转膜1. Load 30 μg of each protein extract or 100 ng of purified protein










