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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
TCR vβ8.3
- 级别:
TCR vβ8.3 Antibody
- 目录编号:
TCR vβ8.3 Antibody
- 克隆性:
单克隆
- 抗原来源:
仓鼠
- 保质期:
TCR vβ8.3 Antibody
- 抗体英文名:
TotalSeq™-A1139 anti-mouse TCR vβ8.3 Antibody
- 抗体名:
TCR vβ8.3 Antibody
- 标记物:
TCR vβ8.3 Antibody
- 宿主:
仓鼠
- 适应物种:
小鼠
- 免疫原:
TCR vβ8.3 Antibody
- 亚型:
TCR vβ8.3 Antibody
- 形态:
TCR vβ8.3 Antibody
- 应用范围:
PG
- 保存条件:
2-8℃
- 浓度:
TCR vβ8.3 Antibody
- 规格:
10 µg
Description :The TCR Vβ8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. 1B3.3 reacts withVβ8.3 only, but not Vβ8.1 or Vβ8.2. Most mouse strains have about 20% Vβ8+ T cells in theperiphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrchaplotype) do not express Vβ8 due to a gene deletion at the Vβ8 loci. Exogenoussuperantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ8 andselectively eliminate those T cells in vivo.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :3.L2 T Cell Clone Expressing the vβ8.3 TCR
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Kersh G, et al. 1998. J Immunol. 161:585-93.
,T 和 B 细胞在免疫系统中展现两种不同功能的识别机制。T 细胞识别细胞膜表面 MHC I 或 II 类分子呈递的抗原肽来识别细胞。而 B 细胞没有选择识别机制。近几年,TCR 样抗体研发工作取得较大进展,许多研究团队都具备制作该抗体能力,研发出了许多针对肿瘤和病毒 T 细胞表位的 TCR 样抗体。能够获得被 T 细胞识别的,纯化后自然折叠 MHC-短肽复合物,是 TCR 样抗体制备关键。MHC-短肽复合物可以通过大肠杆菌表达获得。大肠杆菌分别表达 MHC 重链和β-球蛋白,在体外折叠后,与短肽
分抗体的运输条件:一般的运输过程需要1~2周的时间,所以是在低温4℃的条件下来完成的。 4℃运输最主要的目的是为了避免反复冻融对抗体活性的损害(如果使用干冰运输,那么收到时抗体是冻起来的,为了分装就需要解冻,这就多了一次冻融的过程),所以运输过程中绝对避免干冰运输。 (5)关于叠氮化钠(sodium azide)的使用: 为了防止微生物污染,叠氮化钠经常被加入到抗体中(终浓度0.02% (w/v))。ProSci的绝大部分抗体已经含有了0.02%
细胞毒性T细胞对靶细胞杀伤的主要机制是通过释放的穿孔素-颗粒酶系统介导的溶靶细胞作用或通过fas/fasL系统介导的细胞凋亡作用。因此,对CTL颗粒酶的胞泌作用分析可反映细胞毒性T细胞功能。该方法操作简便且无需靶细胞作为指示细胞。 一、基本原理 应用直接针对TCR的单克隆抗体,模拟特异性靶细胞的作用,诱导CTL细胞内颗粒丝氨酸酯酶等的释放。抗TCR可与CTL细胞表面TCR的α和β链及CD3复合物特异性结合。该法基本过程是,先用单克隆抗体包被微孔板,然后加入CTL或T










