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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD1d (CD1.1, Ly-38)
- 级别:
CD1d (CD1.1, Ly-38) Antibody
- 目录编号:
CD1d (CD1.1, Ly-38) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD1d (CD1.1, Ly-38) Antibody
- 抗体英文名:
Biotin anti-mouse CD1d (CD1.1, Ly-38) Antibody
- 抗体名:
CD1d (CD1.1, Ly-38) Antibody
- 标记物:
CD1d (CD1.1, Ly-38) Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD1d (CD1.1, Ly-38) Antibody
- 亚型:
CD1d (CD1.1, Ly-38) Antibody
- 形态:
CD1d (CD1.1, Ly-38) Antibody
- 应用范围:
FC, IHC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
50 µg/ 500 µg
| 规格: | 50 µg | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 500 µg | 产品价格: | ¥5000.0 |
Description :CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structuralsimilarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans,CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III(CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broadtissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes.CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. Ithas been shown that PKCδ is a critical regulator of CD1d-mediated antigen presentation.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :FC - Quality tested、IHC - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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文献和实验1. Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
2. Brozovic S, et al. 2004. Nat. Med. 10:535.
3. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
4. Jiang J, et al. 2012. PLoS One. 7:47487. PubMed
Product Citations:
1. Kolan S, et al. 2015. Biochem Biophys Res Commun. 460:645. PubMed
2. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
3. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
4. Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed
生物素亲和素(又称抗生物素)系统(biotin avidin system,BAS)标记抗体的技术是 20 世纪 70 年代后期发展起来的一种新的免疫检测方法。 由于亲和素与生物素间的亲和力极强,结合迅速,且极其稳定,生物素标记抗体和酶的标记率高,又不影响蛋白的活性,使 BAS 标记技术比常规酶联免疫、放射免疫及荧光免疫技术有着更高的灵敏度,为微量抗原、抗体的检测开辟了新的途径。近年来,在 BAS 检测中多用链霉亲和素“streptavidin,SA”,它是链霉菌培养过程中的分泌物,完整的链霉
1、上样量80-100太大了,但条带出来很浅,是不是定量有问题或者抗体有问题 2、背景太高,增加封闭时间和洗膜次数 3、杂带很多,抗体特异性不高 erik 我最近做过CST的p-p38,1:800稀释,效果不错。5%牛奶封闭90min,抗体孵育过夜。 lzmclixixi http://immunotech.dxy.cn/bbs/topic/11443307?ppg=24 推荐
本法可使抗体或其它蛋白质的ε-氨基通过手臂与酰化的生物素共价结合。其后,生物素化的分子可应用酶标-亲和素或荧光染料-链霉亲和素复合物来检测。小分子的水溶性生物素对细菌蛋白链霉亲和素具有高度亲和力是本法的设计基础。 NHSB:N-羟基琥珀酰亚胺生物素(有商品供应) 0.1 mol/L 碳酸氢钠缓冲液,pH 8.0 双蒸水(注意去除游离氨基),用以配制碳酸氢钠缓冲液或硼酸缓冲液 DMSO(二甲亚砜,有毒试剂) 1 mol/L NH4Cl 叠氮钠(有毒试剂) PBS










