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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
IL-4
- 级别:
mouse IL-4 Antibody
- 目录编号:
mouse IL-4 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
mouse IL-4 Antibody
- 抗体英文名:
PerCP/Cyanine5.5 anti-mouse IL-4 Antibody
- 抗体名:
IL-4 抗体
- 标记物:
mouse IL-4 Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
mouse IL-4 Antibody
- 亚型:
mouse IL-4 Antibody
- 形态:
mouse IL-4 Antibody
- 应用范围:
ICFC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2200.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5700.0 |
Description :IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is apotent lymphoid cell growth factor which stimulates the growth and activation of certain B cellsand T cells. IL-4 is important for regulation of T helper subset development.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Partially purified native mouse IL-4
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µgper million cells in 100 µl volume. It is recommended that the reagent be titrated for optimalperformance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
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文献和实验1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISACapture)
6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:SpringerVerlag. (IHC)
8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
11. Lawson BR, et al. 2007. J. Immunol. 178:5366.
12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
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Product Citations:
1. Makker P, et al. 2017. PLoS One. 10.1371/journal.pone.0170814. PubMed
2. Zhao Y, et al. 2015. PLoS One. 10: 0134797. PubMed
3. Cai W, et al. 2020. J Immunol Res. 2019:2835256. PubMed
4. Nakatsuji T, et al. 2021. Nat Med. 27:700. PubMed
5. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
6. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
7. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
8. Nakornpakdee Y, et al. 2018. Asian Pac J Allergy Immunol. 36:265. PubMed
9. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
10. Tzeng TT, et al. 2022. NPJ Vaccines. 7:60. PubMed
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大部分老师做流式实验时,会用到空白对照、单阳对照、同型对照、生物学对照等,用到 FMO 对照的比较少。尽管如此,FMO 对照在设置圈门时仍具有重要的参考价值。 一方面,FMO 对照有助于精确地设置阴阳性群体界限。我们在做多色实验时,可能会出现某些通道信号难以区分的情况,主要是因为其他某个荧光对该通道的干扰很大。FMO 对照(Fluorescence Minus One,荧光扣除一)减少了其中一个通道的荧光抗体,能够帮助衡量其它通道的荧光漏溢情况。 如下图所示,PerCP/Cyanine
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